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A25743 ™ SYBR® Green Master Mix
产品名称:A25743 ™ SYBR® Green Master Mix
产品型号: A25743
品牌:
产品单价: 1.00
日期:2019-10-12
A25743 ™ SYBR® Green Master Mix的详细资料

货号: A25743

 

规格  
Detection Method: SYBR
Platform: 7500 Fast Dx System, Real-Time PCR Systems or Thermal Cyclers, 7300 System, 7500 Fast System, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Bio-Rad CFX Connect™, 7900HT Fast System, 7700 System, StepOne™ System, StepOnePlus™ System
Reaction Speed: Fast or Standard
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: Dual-Lock Taq DNA Polymerase
Product Size: 50 mL
Shipping Condition: Wet Ice
Number of Reactions: 5000 reactions
  内容及储存  
The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 X 50 mL tube, sufficient for 5000 20-µL reactions (10 µL Master Mix per reaction).
Store at 2–8°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
  描述   PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range

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